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Team:Bielefeld-Germany/Test
From 2012.igem.org
Woche 1
Week 1 (04/30 - 05/06/12)
- Start of our WET LAB time.
weekly seminar:
- Do we want to order strains of Trametes versicolor and Trametes villosa?
- Gathering information about signal sequences in yeast
- Decision to create a database, so that we can easily number and inscribe our lab results
- Decision to arrange a summer school for pupils in their last year before the final exams
- Discussion about how to meet a member of the german Bundestag (the german parliament)
Monday April 30th
Production
These are the protocols for the cultivations and the downstream processing.
Before one is able to work with a cell-free system based on biological material, the needed proteins have to be produced and purified first. These methods and the ones we used to characterize BioBricks in vivo are presented in this section. Used methods: Cultivations in shaking flasks and bioreactor; protein clean-up from medium, periplasm, whole cell and inclusion bodies; UF / DF; IEX; Ni-NTA columns and chromatography; recrystallization and immobilization of S-layer proteins. read more
Analytics
Protocols for the analytical methods we used.
DNA and proteins are very small and cannot be seen by the naked eye. To control the success and the results of your upstream and downstream processes, analytical methods are necessary that give reliable results to make DNA or proteins in any way visible for you. The analytical methods we used in our project can be found in this section. Used methods: Fluorescence measurement; SDS-PAGE; MALDI-TOF; HPLC; LC-ESI-qTOF-MS/MS; molecular beacons; extraction. read more
Immobilization
Here you can find out, how we immobilized the produced enzymes.
Coming soon read more
Material
Chemicals, enzymes and kits we used in our lab work.
Chemical and biological reactions need defined conditions to work as expected. The chemicals, enzymes, kits, buffers and media we used in our project are listed in this section. read more
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