Team:GeorgiaState

The 2012 Georgia State University iGEM Team strives to standardize the glyceraldehyde-3-phosphate dehydrogenase (pGAP) promoter shuttle vector to be used in the methylotrophic yeast Pichia pastoris. Creating a standardized expression system within this eukaryotic organism allows the expression of a variety of proteins that can be used for many biological purposes. P. pastoris, in comparison to the conventional recombinant host E. coli, is an ideal choice for the expression of complex proteins due to its ability to perform post-translational modifications and provide a secretion system for these molecules. Modifying the pGAP vector to be used with the iGEM standard enables Georgia State and other teams to utilize P. pastoris as an expression host. In the future, we also plan to standardize the pPic9 shuttle vector to the iGEM standard.