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From 2012.igem.org
Digestion protocol
- 8 uL DNA linearized plasmid Backbone (25 ng/uL) - 1uL PstI - 1uL EcoRI - 2.5 uL H Buffer - X uL Water
TOTAL 25 uL
-- The digestion mixture is kept for 3 hours at 37 ° C
-- The mixture is kept for 20 minutes at 80ºC
After those 20 minutes, the plasmid pSB1C3 is ready to use.
