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From 2012.igem.org

Ordered gBlock fragments are nearly here!

Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:

• +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
• -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
• +T7 promoter Heme oxygenase
• -T7 promoter Heme oxygenase

Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.

BioBrick name	Fragment(s)
T7 Heme oxygenase	Hemo_T7_A + Hemo_B + Plasmid
Heme oxygenase (T7 minus)	Hemo_A + Hemo_B + Plasmid
T7 Agrobacterium	Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
Agrobacterium (T7 minus)	Agro_A + Agro_B + Agro_C + Agro_D + Agro_E

Once the Deinococcus Radiodurans fragments arrive we will assemble them using Gibson assembly. Due to the large sequence of the D. radiodurans bacteriophytochrome, the fragments will be assembled without a T7 promoter. The designed sequences of the 5 fragments can be viewed here

BioBrick name	Fragment(s)
Deinococcus radiodurans bacteriophytochrome	Deino_A + Deino_B + Deino_C + Deino_D + Deino_E + Plasmid