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Team:Macquarie Australia/Protocols/ArrivalofGBlocks
From 2012.igem.org
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.
Biobrick Name Part(s) for BioBrick T7 Heme oxygenase Plasmid Hemo_T7_A Hemo_B Heme oxygenase (T7 minus) Plasmid Hemo_A Hemo_B T7 Agro Plasmid Agro_T7_A Agro_B Agro_C Agro_D Agro_E Agro (T7 minus) Plasmid Agro_A Agro_B Agro_C Agro_D Agro_E BioBrick name Fragment(s) T7 Heme oxygenase Hemo_T7_A + Hemo_B + Plasmid Heme oxygenase (T7 minus) Hemo_A + Hemo_B + Plasmid T7 Agro bacterium Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid Agrobacterium (T7 minus) Agro_A + Agro_B + Agro_C + Agro_D + Agro_E
