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Team:Wageningen UR/Protocol
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Top
Home
Team
The Team
Team Registration
Project
1. Introduction
2. PnA System
3. Virus-Like Particles
CCMV
HepB
Polerovirus
Natural BioBrick
4. Outside Modification
5. Inside Modification
6. Detection of VLPs
7. Applications
8. The Constructor
9. Final Overview
Human Practices
Human Practices Home
1. Mini Symposium
2. Visiting Secondary Schools
3. Discovery Festival
Communication Science
4. Stakeholders
5. Munich CAS Conference
Safety
Introduction
1. General safety
2. Virus-related safety
3. Regulations
4. Safety suggestions
5. Safety of applications
Modeling
Human Body Model
Tertiary folding prediction
VLP assembly model
Attributions
Parts
Notebook
Journal
Protocols
Contents
1
Protocol
1.1
Medium & Buffer recipies
1.2
CCMV Coat Protein VLP formation
1.3
Hepatitis B Coat Protein VLP formation
1.4
PoLeRo Coat Protein VLP formation
Protocol
Medium & Buffer recipies
LB medium
SOB medium
Disassembly buffer
Dialysis buffer 10x
Reassembly buffer
Virus buffer
CCMV Coat Protein VLP formation
Growing culture
Dialysis of the VLPs
Purifing the VLPs
Hepatitis B Coat Protein VLP formation
PoLeRo Coat Protein VLP formation
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