Team:Tokyo Tech
From 2012.igem.org
Contents |
Story
Love is like a butterfly.It goes where it pleases and it pleases where it goes. Summer comes and the season of love comes! However, we want to do something special in this summer.We decide to make E.coli love like human! In order to achieve this idea, we focused on cell-cell communication between two E.coli. We are aiming for that by two types of cell-cell communication signals, E.Coli will be starring in 「Romeo and Juliet」which is among Shakespeare's most popular plays.
Chemical synthesis of PHA
JULIET: O Romeo, Romeo! wherefore art thou Romeo? Deny thy father and refuse thy name; Or, if thou wilt not, be but sworn my love, And I'll no longer be a Capulet.
ROMEO: Shall I hear more, or shall I speak at this?
JULIET: 'Tis but thy name that is my enemy; Thou art thyself, though not a Montague. What's Montague? it is nor hand, nor foot, Nor arm, nor face, nor any other part Belonging to a man. O, be some other name! What's in a name? that which we call a rose By any other name would smell as sweet.
PHA is a kind of bio-plastic produced by microbes.It's standard extract can be got after being dissolved in chloroform. It is still a technical challenge to shape PHA because of it's fragility, so we thought of one way to use PHA without shaping it. It is PHA's property of water-repellent. According to the equation of Yang and Wenzel, the bigger the surface area is, the stronger the water-repellency can get. Hence, our goal is to stick PHA on weak water-repellent membrane in order to achieve stronger water-repellency.