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From 2012.igem.org

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Contents 1 Protocols 1.1 DNA Purification 1.2 DNA Assessment 1.3 PCR Methods 1.4 Plasmid Insertion 1.5 Plasmid Editing 1.6 Maintenance Tasks

Protocols

DNA Purification

• Miniprep
• Gel Extraction

DNA Assessment

• Gel Electrophoresis
• DNA Quantification
• Sequencing
• PCR Clean Up

PCR Methods

• Vent PCR
• Phusion PCR
• Colony PCR

Plasmid Insertion

• Transformation of E. coli
• Transformation of Shewanella
• Conjugation

Plasmid Editing

• Digestion Reaction
• Dephosphorylation
• Ligation Reaction
• Gibson Assembly

Maintenance Tasks

• Making LB
• Making Super Optimal Broths
• Making Plates
• Autoclave
• Preparing Glycerol Stocks
• Preparing Overnight Cultures
• Preparing Electrocompetent Cells