• Take a 6 µl aliquote of the DNA and put back the main DNA tube in the fridge.
• Go to the room by the E.Coli lab (LBTM, not on Friday morning!) with:
  • The 6 µl aliquote
  • A 10 µl pipet
  • Optionally, the buffer you used for DNA elution (there might be some next to the machine).
• The machine is the NanoDrop Spectrophotometer.
• On the computer, click on "Nucleic Acid".
• Put a 2 µl drop of (nuclease-free) water on the machine's tip as you are asked to and measure.
• Clean tips (both sides) with a quarter of tissue.
• Add 2 µl of the buffer you use and click on "Blank".
• Clean tips (both sides).
• Add 2 µl of your DNA sample and click "Measure".
• Clean tips (both sides) with a tissue.
• Take 2 measurements per sample (for averaging).
• Print the report when you are done
• Click on exit.

The important numbers are:

• 260/280 ratio, must be > 1.8
• 260/230 ratio, must be > 2 (too big, > 2.5? , might mean too much salts)
• Of course the DNA concentration.