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Team:BostonU/Clotho
From 2012.igem.org
Clotho and Eugene
Clotho
Spreadit Oligo
Spreadit oligos is an app that allows users to browse and input oligos from and into the data base. We used this app to add on additional oligos one at a time aside from spreadsheets of primers that were added by Bull Trowell. Spreadit Oligos does what Bull Trowell does on a smaller scale.
Clotho
Bull Trowell
Bull Trowel is a very useful app for adding large amounts of Parts, Oligos, Vectors, Plasmids and etc from an excel sheet to Clotho`s inventory. In our case, we had a list of almost 200 primers that we designed in an Excel sheet that we wanted to include in our inventory. All of them could be added much faster and with lower mistake rate by using Bull Trowel. Below, there is an example of the interface of the app while we were adding our oligos and the Excel sheet from which we took the oligo sequences.
Also, the description section helps to give meaning to the nickname and makes the inventory more user-friendly.
Clotho
Sequence Viewer
Sequence Viewer is a very useful app to analyze sequences that are in the inventory . It allows us not only to view the sequences of DNA, but also to interact with it by highlighting specific portions that we are interested in. For example, we used sequence viewer to find and highlight restriction and fusion sites within the sequence.
