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From 2012.igem.org

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Contents 1 Protocols 1.1 DNA Extraction 1.2 PCR Methods 1.3 Plasmid Insertion 1.4 DNA Assessment 1.5 Plasmid Editing 1.6 Other 1.7 Maintenance Tasks

Protocols

DNA Extraction

• Miniprep
• Gel Purification

PCR Methods

• Vent PCR
• Phusion PCR
• Colony PCR

Plasmid Insertion

• Transformation of E. coli
• Transformation of Shewanella
• Conjugation

DNA Assessment

• Gel Electrophoresis
• DNA Quantification
• Sequencing

Plasmid Editing

• Digestion Reaction
• Ligation Reaction
• Gibson Assembly

Other

• Dephosphorylation
• PCR Clean Up

Maintenance Tasks

• Making LB
• Autoclave
• Making Super Optimal Broths
• Preparing Glycerol Stocks
• Preparing Overnight Cultures