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Contents 1 Subprojects 2 Protocols

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Subprojects

DO NOT DO ANY EDITING ON THE BELOW LINKS. THE BETTER VERSIONS ARE THE ABOVE. THESE ARE ONLY STILL HERE FOR REFERENCE AS A "JUST IN CASE"

• Arsenic reporter -- test a system for Shewanella detecting arsenates and activating the Mtr pathway to produce an electrical current
• nah operon -- an operon that converts napthalene into salicylate, allowing it to be detected by the salicylate reporter.
• Salicylate reporter -- a system for Shewanella detecting salicylate and activating the Mtr pathway to produce an electrical current

Protocols

• Miniprep
• Vent PCR
• Phusion PCR
• Colony PCR
• Gel Electrophoresis
• Gel Purification
• DNA Quantification
• Digestion Reaction
• Dephosphorylation
• PCR Clean Up
• Ligation Reaction
• Transformation of E. coli
• Transformation of Shewanella
• Sequencing
• Gibson Assembly
• Making LB
• Autoclave
• Making Super Optimal Broths