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Team:Cambridge/Week 4
From 2012.igem.org
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Contents |
Monday
Scandal in the lab! It appears that someone at Caltech has edited our wiki, initially inserting the word 'chicken' into our project page. Then they broke our Java script - potentially accidentally. Easily rectified, but still irritating, especially since the project description deadline was yesterday. Have we been the victim of a not-so-droll ivy league-ish prank? We've emailed their project supervisor, so hopefully it shouldn't happen again.
One night seems to have been too little time to accurately determine if the transformation worked, as we now have lots of e.coli colonies on the plates. The bacillus plates have not been so successful, but the plasmid was not optimized to bacillus, potentially explaining their failure. We will need to make sure that we include more controls in future to work out where our experiments fail, if they should fail.
In any case, we were able to induce the pBAD promoter with arabinose in the e.coli cells. Results can be seen in the lab book page.
Additionally, Andreas managed to finish his Arduino device. It now gives a response that is proportional to the ratio of the light intensities on the two light dependent resistors. By using a couple of coloured filters over the top of these resistors, we can measure the necessary spectral properties of our engineered luciferases. All the engineers need to do now is come up with a user interface.
