"
Team:Cambridge/Week 3/Lab book
From 2012.igem.org
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions | Sponsors |
|---|
| Diary | Lab book |
|---|
Contents |
Monday
Making bacillus competent
Bacillus salts *10 made up:
- (NH4)2SO4: 20g
- KH2PO4 (anhydrous): 60g
- Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
- KH2PO4 60g
- Tri-sodium citrate 10g
- MgSO4•7H2O 2g
- Added distilled water to make 1000mL
- Autoclaved
Medium A base *10 made up:
- Yeast extract 10g
- Casamino acids 2g
- Added distilled water to make 900mL
- Autoclaved
bacillus strain 168 streaked out and grown on plate overnight.
Tuesday
Making bacillus competent
Wednesday
Making bacillus competent
Thursday
Test transformation of frozen bacillus stocks
Friday
Transformation of bacillus with lux genes from 2010
