Team:Nevada/Protocols

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Phusion PCR

Thermocycling conditions:
Initial Denaturation: 98°C for 30 seconds
25-35 cycles:

  • 98°C for 10 seconds
  • 55°C, 60°C, 65°C for 15 seconds
  • 72°C for 15 seconds

Final Extension: 72°C for 5 minutes

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Qiagen Miniprep kit

www.qiagen.com/hb/qiaprepminiprep