Team:SJTU-BioX-Shanghai

This year, SJTU-BioX-Shanghai iGEM team is trying to build a “factory” on E.coli’s membrane, where enzyme assemblies can be manipulated so that biochemical reactions can be accelerated and further controlled.

We aimed at constructing a set of protein assemblies on E.coli inner membrane as carriers of various enzymes. Distinct from previous synthetic scaffold system, our device limits the reaction space to a two-dimensional surface. In such system, the membrane functions as an extensive scaffold for proteins to anchor without limitation of scaffold amount. Membrane as scaffold also has privilege in receiving external and internal regulating signals. Based on Membrane Scaffold, we built two universal devices.

In Membrane Accelerator device, by gathering downstream enzymes through interacting protein domains, the reaction can be accelerated sharply. Production of fatty acid was enhanced by more than 24 fold through recruiting membrane accelerator system, which has a promising application prospect in biofuel production.

In Membrane Rudder device, we successfully changed the direction of Violacein synthetic pathway through light signal.

ü The honors we won: Gold Medal, Best New BioBrick Part or Device Engineered (BBa_K771001) and Regional Winner at Asia Jamboree; Advanced to World Championship.

ü The breakthrough we made: Redefinition of scaffold in Synthetic Biology by recruiting E.coli’s inner membrane as a natural two-dimensional scaffold.

ü The system we built: 6 membrane proteins orderly organized on the inner membrane of E.coli, the efficiency of which has been proved by fluorescence complementation assay and biosynthesis experiment.

ü The device we created – Membrane Accelerator: A universal tool that serves to accelerate biochemical reactions in E.coli; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type E.coli and 9 fold compared to that with overexpressed cytoplasmic enzymes.

ü device we created – Membrane Rudder: A universal tool used to dynamically and artificially control biochemical reactions in E.coli; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched.

ü New direction we proposed: The application of scaffold system in accelerating biodegradation pathway using our Membrane Accelerator.

ü Parts we submitted: 42 well-characterized parts that could either be used directly or serve as a universal tool readily for potential scientific or engineering use.

ü A club we established – BioCraft: The headquarter of our human practice programs, having come a long way in propagandizing Synthetic Biology and iGEM. Warmly-received activities have been held in and outside the campus. Several celebrities in different fields have shown support for us, laying a cornerstone for our future development.