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Team:Leicester/Project
From 2012.igem.org
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Overall project
Our project is about trying to reduce the waste going to landfill by engineering a bacteria to degrade polystyrene. Some bacteria have been found to form biofilms on polystyrene, indicating that polystyrene may be being degraded. We have several different parts to our project, including a citizen science experiment (CSE).
Project Details
Part 2
The Experiments
Plating out the returned Citizen Science Experiment kits
The first part involves halving the expanded polystyrene lengthways (EPS) with sterilized scissors in our returned citizen science experiment (CSE) kits so that we'll still have some of each sample left should we need it. First we need to do a practice with unused EPS strips (control and test). Pour/pipette 20ml LB agar into a petri dish and allow to set. Put line down middle in permanent marker USING RULER! Add PBS to the half strip of EPS and scrape with spreader, before spreading onto the left half of the agar (or side labeled as 'swab'). Try to swab any mud off the strip if possible. Then place the half strip in the centre of the other half labeled 'strip'. Leave for a week at room temperature on the lab bench and examine agar for colonies. The other half of the strip should be put back into cold storage for future use.
--Luke Thompson 07:51, 9 July 2012 (CDT)
