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Team:Leicester/Project
From 2012.igem.org
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Overall project
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Project Details
Part 2
The Experiments
Plating out the returned Citizen Science Experiment kits
The first part involves halving the expanded polystyrene (EPS) in our returned citizen science experiment (CSE) kits so that we'll still have some of each sample left should we need it, after first doing a practice with unused EPS strips (control and test). Pour/pipette 20ml LB agar into a petri dish and allow to set. Put line down middle in permanent marker USING RULER! swab whole half strip of EPS and spread onto the left half of the agar (or side labeled as 'swab'). Try to swab any mud off the strip if possible. Then place the half strip in the centre of the other half labeled 'strip'. Leave for a week at 37 degrees and examine agar for colonies- identify if you have time using tests such as catalase activity, gram positive/negative etc. The other half of the strip should be put back into cold storage for future use.
--Luke Thompson 07:51, 9 July 2012 (CDT)
