We successfully produced purified and identified active laccases of
E. coli BL21(CueO named ECOL),
Bacillus pumilus DSM 27 laccase (CotA named BPUL),
Bacillus halodurans C-125 laccase (Lbh1 named BHAL) and
Thermus thermophilus HB27 (tthL named TTHL).
ECOL/coli Results
BPUL/pumi Results
TTHL/thermo Results
BHAL/halo Results
We successfully designed a shuttle vector for site-directed integration in
P. pastoris which allows the production and secretion of a protein of interest.
Results
Successful characterization of the activity of all four bacterial laccases regarding the oxidization of ABTS under different conditions.
ECOL/coli#Activity_Analysis_of_ECOL Results
BPUL/pumi#Activity_analysis_of_BPUL Results
TTHL/thermo#Activity_analysis_of_TTHL Results
BHAL/halo#Activity_Analysis_of_BHAL Results
We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.
Results
We successfully immobilized
Trametes versicolor-laccase (named TVEL0),
E. coli BL21-laccase (CueO named ECOL) and
Bacillus pumilus DSM 27-laccase (CotA named BPUL) and measured their activity.
TVEL0/immo Results
ECOL/immo Results
BPUL/immo Results
We successfully improved a BioBrick by clarifying its true function.
[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
We successfully created our own database to organize our samples.
Results
We successfully created a model which simulates the oxidation of ABTS.
Results
We conducted a feasibility study of our project with sewage plant experts.
Results
We did an outstanding outreach work.
Results
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