Utk knoxville august

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8.20.12
Digested PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc with EcoRI & PstI for confirmation.
Digested J63010 + Adh + mOrange with SpeI and PstI. Digested J63010 + pIRES + GFP + cyc with XbaI and PstI.

8.21.12
Ran gel of check digestions for PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc. All three constructs were confirmed.
Ligated pIRES + GFP + cyc into J63010 + Adh + mOrange. Did 1:0, 1:10, and 1:30.
Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc.

8.26.12
Amplified GFP + Cyc.
Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc.

8.28.12
Digested :
J63010 + Adh with EcoRI and PstI to confirm construct.
J63010 + Adh with SpeI and PstI.
PSB1C3+ KanR with XbaI and PstI.
PSB1C3 + p150 with SpeI and PstI.

Amplified GFP + Cyc with XbaI and PstI.