Team:Lethbridge/notebook

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Notebook

Our notebooks document the University of Lethbridge's iGEM Team through their scientific journey

By coupling carbon capture with acetic acid and biosurfactant production, carbonate oil deposits can be mined with reduced greenhouse gas emissions. The use of carbon fixation to feed downstream systems can be tailored for use as a module in many applications requiring inexpensive methods for fueling biological systems. CAB extraction will be suitable for large-scale bioreactors, providing an alternative, inexpensive, and environmentally sustainable method for MEOR from Alberta’s oil deposits. Furthermore, developing the carbon capture module will be of interest in oil extraction strategies using steam, as it will help with the mitigation of CO2 release caused by steam production using for example natural gas.

References

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4. World Energy Outlook 2010 - Executive Summary. (International Energy Agency, 2010).

5. MicroPro-Gmbh. MEOR case study - oil field “Doebern” (Germany), (2011).

6. Sen, R. Biotechnology in petroleum recovery: The microbial EOR. Progress in Energy and Combustion Science 34, 714-724, doi:10.1016/j.pecs.2008.05.001 (2008).

7. Niederholtmeyer, H., Wolfstaedter, B. T., Savage, D. F., Silver, P. A. & Way, J. C. Engineering Cyanobacteria To Synthesize and Export Hydrophilic Products. Applied and Environmental Microbiology 76, 3462-3466, doi:10.1128/aem.00202-10 (2010).

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15. Nakano, S., Fukaya, M. & Horinouchi, S. Putative ABC Transporter Responsible for Acetic Acid Resistance in Acetobacter aceti. Applied and Environmental Microbiology 72, 497-505, doi:10.1128/aem.72.1.497-505.2006 (2006).

16. Wang, Q. et al. Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery. Biotechnology and Bioengineering 98, 842-853, doi:10.1002/bit.21462 (2007).

17. Patel, R. M. & Desai, A. J. Biosurfactant production by Pseudomonas aeruginosa GS3 from molasses. Letters in Applied Microbiology 25, 91-94, doi:10.1046/j.1472-765X.1997.00172.x (1997).

18. iGEM2011. Results Summary, (2011).

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20. Ohno, H. et al. Synthetic RNA-protein complex shaped like an equilateral triangle. Nature Nanotechnology 6, 115-119, doi:10.1038/nnano.2010.268 (2011).

21. Zhu, C. & Ye, Q. [Selection of acetate-tolerant mutants from Escherichia coli DH5alpha and the metabolic properties of mutant DA19]. Wei Sheng Wu Xue Bao 43, 460-465 (2003).

22. Ochsner, U. A., Fiechter, A. & Reiser, J. Isolation, Characterization, and Expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB Genes Encoding a Rhamnosyl Transferase Involved in Rhamnolipid Biosurfactant Synthesis. Journal of Biological Chemistry 269, 19787-19795 (1994).

23. iGEM2011. BioBricks, (2011).

24. Ohlendorf, R., Vidavski, R. R., Eldar, A., Moffat, K. & Möglich, A. From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. Journal of Molecular Biology 416, 534-542, doi:10.1016/j.jmb.2012.01.001 (2012).

25. Flynn, J. M., Neher, S. B., Kim, Y.-I., Sauer, R. T. & Baker, T. A. Proteomic Discovery of Cellular Substrates of the ClpXP Protease Reveals Five Classes of ClpX-Recognition Signals. Molecular Cell 11, 671-683, doi:10.1016/s1097-2765(03)00060-1 (2003).