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Contents 1 Hello 2 July 2.1 4th 2.2 5th 2.3 6th

Hello

We are team HokkaidoU Japan! Today we learn and start to edit wiki. (>ω<)　
Dear Mr.Ortiz, I saw the help page which you edited.
hola!

July

4th

Transformation

• Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
  • Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43)
    • T7:21hrs Others:20hrs

5th

Transformation

• Transformation of K346007(Ag43)in DH5α.
  • Cultivated on LBC in 21hrs.

Single colony isolation

• Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
  • Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins
    • BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.

6th

Liquid culture

• Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
  • LB : 1ml is for glycerol stocks and 2ml is for mini-prep.

Single colony isolation

• Single colony isolation of K346007(Ag43).