Team:Berkeley/Attributions

Wetlab

Many of our basic parts (promoters, terminators, and fluorescent proteins) are courtesy of the Dueber Lab at UC Berkeley, our host this year. The iGEM team chose the targeting proteins and sequences from the the Yeast GFP Database created by the O'Shea and Weissman Labs at UCSF, and either PCRed them from genomic DNA or synthesized them via gene synthesis. The iGEM team then combined these parts with the basic parts provided by the Dueber Lab to build all the devices they utilized.

All the device cloning was done by the iGEM team. This involved many Golden Gate Assembly reactions, minipreps, transformations, digestions, yeast integrations, etc. While some of the Golden Gate backbones were provided by the Dueber Lab, many of the parts specific to MiCode project, specifically the backbones for multigene and full MiCode assembly, were designed and cloned by the iGEM team.

The leucine zipper sequences were courtesy of the Keating Lab at MIT, in a collaborative effort to identify an orthogonal interaction set. With the sequences provided, the iGEM team constructed the leucine zippers via gene synthesis, combined them into their MiCode construction scheme, and assayed them in yeast.

Computational

On the software side, the iGEM team wrote the MATLAB code, constructed the Cell Profiler pipeline for automated image analysis, and tested it using images generated by the iGEM team.


Website

With their permission, we began with the template of the Berkeley 2011 iGEM team, and tweaked it to our needs this year.