Team:Berkeley/Attributions

Wetlab

Many of our basic parts (promoters, terminators, and fluorescent proteins) are courtesy of the Dueber Lab at UC Berkeley, our host this year. The iGEM team chose the targeting proteins and sequences from the the Yeast GFP Database created by the O'Shea and Weissman Labs at UCSF, and either PCRed them from genomic DNA or synthesized them via gene synthesis. The iGEM team then combined these parts with the basic parts provided by the Dueber Lab to build all the devices they utilized.

All the device cloning was done by the iGEM team. This involved many Golden Gate reactions, minipreps, transformations, digestions, yeast integrations, etc.

The leucine zipper sequences were courtesy of the Keating Lab at MIT, in a collaborative effort to identify an orthogonal interaction set. The iGEM team constructed the MiCoded leucine zippers and assayed them.

The Golden Gate assembly of our multigene and MiCode cassettes was designed and performed by the iGEM team.


Computational

On the software side, the iGEM team wrote the MATLAB code, constructed the Cell Profiler pipeline for automated image analysis, and tested it using images generated by the iGEM team.


Website

With their permission, we began with the template of the Berkeley 2011 iGEM team, and tweaked it to our needs this year.