Team:HUST-China/Project/Protocols

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HUST CHINA



E.Z.N.A.™ High Performance Plasmid Mini Kit I (http://www.omegabiotek.com/files/resource/Handbook/09324200.pdf)
E.Z.N.A.® Gel Extraction Kit (http://www.omegabiotek.com/files/resource/Handbook/44320600.pdf)
TIANprep Mini Plasmid Kit (http://www.tiangen.com/asset/imsupload/up0168548001342417710.pdf)
TIANgel Midi Purification Kit (http://www.tiangen.com/asset/imsupload/up0677301001342419001.pdf)
DNA Double Digestion Protocol (https://static.igem.org/mediawiki/2012/8/84/DNA_double_digestion_protocol.pdf)
Immunofluorescence Protocol (https://static.igem.org/mediawiki/2012/2/25/Immunofluorescence_protocol.pdf)
PCR Protocol (https://static.igem.org/mediawiki/2012/3/37/PCR_protocol.pdf)
Pichia Yeast Transformation Protocol (https://static.igem.org/mediawiki/2012/0/02/Pichia_Yeast_Transformation_protocol.pdf)
Ligation of Insert DNA into Plasmid Vector DNA Protocol (https://static.igem.org/mediawiki/2012/4/46/Protocol_for_ligation_of_insert_DNA_into_plasmid_vector_DNA.pdf)
Yeast DNA Rapid Isolation Protocol (https://static.igem.org/mediawiki/2012/1/1d/Protocol_for_Rapid_Isolation_of_Yeast_DNA.pdf) Competent Cells Preparation Protocol (https://static.igem.org/mediawiki/2012/3/3d/Protocol_for_preparation_of_competent_cells.pdf)
SDS-PAGE Protocol (https://static.igem.org/mediawiki/2012/9/96/SDS-PAGE_Protocol.pdf)
Transformation Protocol (https://static.igem.org/mediawiki/2012/7/75/Transformation_protocol.pdf)
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