Team:UANL Mty-Mexico/Notebook

Cloning Strategy

Fusion proteins

We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.

Figure 1. Cloning strategy for contructions of fusion proteins.