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Team:Michigan/Notebook/Protocols

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Modify HbiF into a Biobrick compatible format

HbiF and LVA degradation tag were ordered from IDT with the following specifications.

EcoRI

XbaI

SpeI

PstI

AgeI

NgoMIV

HbiF possesses an N-terminus in the (truncated) RFC10 format :

GAATTC GCGGCCGC T TCTAG [ATG]

No extra amino acids are created by this prefix, in comparison to the standard RFC25 prefix, which creates a serine and glycine. The suffix proceeds as follows:

ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG

Prepare FimE for protein expression

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