Team:UT-Tokyo/LabWork/RegularMethods
From 2012.igem.org
Regular Methods
Assembly parts
Digest
Materials
Protocol
Ligation
Materials
- Vector DNA
- Insert DNA
- 2x Ligation Mix
Protocol
1.Make reaction liquid
- MilliQ up to 20uL
- 10uL 2x Ligation Mix
- Vector DNA
- Insert DNA
2.Incubation at 16 °C for 15-30 min.
Transformation
Purification of DNA
Reagents