Successfully cloned and submitted 32 biobricks into the registry
Obtained WbbC gene from BL21 genome
Created GlycoBase; a database containing a list of >100 glycosyltransferase enzymes
from E.coli strains to demonstrate the vast linkages we could achieve only with E.coli enzymes
Created GlycoWeb, an interface for an online user to access Glycobase with a
polysaccharide in mind and the database searches and finds the enzymes you’d use to make it.
Created GlycoApp; an application for a handheld device to access the Glycobase from
wherever the consumer is when they realise they need a polysaccharide ordering!
Considered human practice approaches and discussed with several experts the human
practice considerations, hosted a human practice panel and discussed with the wider community at Café Scientifique.
Discussed our project with various businesses including Shell, Ginsters etc. and we
received letters of support from DSTL, ISCA Biochemical Ltd, Avon.
Demonstrated that….. Successfully expressed this polysaccharide….. (to be cont….)
Made promoter-RBS-gene-terminator constructs for each gene
Failures
A correct mass analysis of the product is essential, but cannot alone confirm the
correct product. The possibility that an incorrect product has been made through incorrect synthesis or post-synthetic regiospecific or enantiospecific rearrangement by
chemical or enzymatic means must be examined and ruled out before the method can be considered reliable.
Synthesis of a previously well characterised small polysaccharide, which can be
physically and also biochemically characterised, to show that the product will act in biological systems as expected. Once a fully characterised polysaccharide is produced, we
would be in a much stronger position to offer novel products and to start to address the exciting possibilities of our method.
Only managed to obtain 7 of 9 fragments for Gibson assembly through PCR.
Didn’t manage to construct operons through BioBrick assembly.
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