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30th july

• Analysis of the Petri dishes after the transformation with the B construction

Electrophoresis of BBa_K274100 and pSB1A2 on a 0.8% Agarose gel.

31th July

• Miniprep of the colonies B1 and B2 that came from the transformation with the B construction.
• Nanovue to measure the concentration of the samples B1, B2 and the control

• Amplification by PCR of the 880bp end of BBa_K098995 contained in the colonies B1, B2 and test on the control and amplification of the whole B construction.

PCR program used: