The steps of the experiments

1. Design of amiRNA Sequences

The target gene we want to silence is PGC-1a which is related to metabolism in several tissues. After inputting the sequence of PGC-1a to the Web MicroRNA Designer, the software will give us several potential amiRNA sequences which have high possibilities to silence the target genes. Although the sequences are picked out based on several principles, we still need to select the sequences by ourselves in the rules: