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Team:Lyon-INSA/datapage
From 2012.igem.org
Revision as of 18:05, 26 September 2012 by Mwolfovski (Talk | contribs)
Interactive pattern of our construction : Toggle switch option
Our parts :
Data about our favorite new parts
1.Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis Constitutive Promoter (Pveg) with the dispersin B gene (dspB). We tried two different genetic backgrounds : the NM522 strain to make test in E. coli and the 168 strain to make test in Bacillus subtilis.2.Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. We determined antibiotic resistance thresholds and transformation efficiencies and compared them to BBa_K802004
3.Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis Constitutive Promoter (Pveg) with the lysostaphin gene. The pBKL28 plasmid was transformed in the NM522 strain to perform tests in E. coli and in the 168 strain to perform tests in Bacillus subtilis.
We've also characterized the following new parts
Main Page: Plac(B. subtilis)-RBS(E. coli)-GFP BBa_K802002 : This part is used to determine if the Plac promoter works or not and especially how it works when it is activated by different concentration of the inducer IPTG. Within this part there is Plac from Bacillus subtilis which is the promoter that induces production of XylR to form a positive biofilm. Then a RBS from E coli is added to finally enable production of GFP which is an easy measurable parameter using the amount of fluorescence.Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. We determined antibiotic resistance thresholds and transformation efficiencies and compared them to BBa_K802003
Main Page: Surfactin generator and biofilm repressor for B. subtilis BBa_K802009: This part can be used to induce surfactin production (an antimicrobial lipopeptide) and to repress the biofilm formation in B. subtilis strains.
