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Daily Lab Notebook

Weeks 1&2:

 

1) Amplification of pvdQ gene using PCR from Pseudomonas aeuriginosa genomic DNA. The designed primers contain the prefix and suffix so that typical digestion for insertion into iGEM’s standard vector can be subsequently performed.


2)Test digestion of amplified pvdQ with EcoRI only, EcoRI & SpeI, PstI, and EcoRI & XbaI.

 

 
3). Constitutive Promoter (pLacI) + Ribosomal Binding Site + LuxR Gene + Double Terminator + LuxR Promoter (J09855) + Ribosomal Binding Site (B0034) + pvdQ Gene + Double Terminator (B0015)

 

4). LuxR Promoter (R1062) + Ribosomal Binding Site, LuxR Gene, Double Terminator, & Ribosomal Binding Site (amplified from K137076) + pvdQ Gene + Double Terminator (B0015)