Team:UT-Tokyo/Notebook

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Notebook

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このページの概要を、簡単に記述。


May

1st week: 5/27-6/2

May 29th

  • Making LB medium

May 30th

  • Making LB Agar
  • Making LB Amp Plates


May 31st

  • Preculture


June

2nd week: 6/3-6/9

June 5th

  • Plating JM109

June 6th

  • preculture

June 7th

  • Measuring OD by spectroscope
  • Meking electrocompetent cells
  • Electroporating of a test plasmid

June 8th

  • Caluculatiing transformation efficiency


3rd week: 6/10-6/16

4th week: 6/17-6/23

5th week: 6/24-6/30

June 26th

  • Making LB tetracycline plate

June 27th

June 28th

  • Making LB kanamycin plate and chloramphenicol plate
  • Transformation with a test plasmid

June 29th

  • Transformation with #1(constitutive promoter) and #11(RBS)

2012/07

6th week: 7/1-7/7

July 3rd

  • Making K solution
  • Colony PCR DNA fragment assumed as fhlA (#6) for cloning

July 4th

  • Miniprep #1, #11

July 5th

  • Making 1% agarose solution
  • Electrophoresis (#6)
  • Digest (#1_EP, #11-_EP)

July 6th

  • Colony PCR (#6)

7th week: 7/8-7/14

July 9th

  • Electrophoresis (#6)

July 10th

  • Transformation
    • #3(RBS medium), #5(d.term), #20(pLac)

July 11th

  • Preculture
    • #3, #5, #20
  • Gel Extraction(#6)

July 12th

  • The continuation of Gel Extraction(#6)
  • Miniprep
    • #3, #5, #20

July 13th

  • Nanodrop (#6).
  • Digestion (#3:E-P, #5:E-P, #20:E-P)
  • Electrophoresis (#3, #5, #20)

July 14th

  • Miniprep (#1,3,5,11,20)
  • Gel extraction (#6)

8th week: 7/15-7/21

July 17th

  • Transformation (#1A2,#3,#5,#20)
  • PCR (parts of 1. and #3,#20(Colony PCR))
  • Incubate #5.

July 18th

  • Miniprep (#1A2,#3,#5,#20)
  • PCR using u.p (#1A2,#3,#5,#20)
  • Colony PCR (#3,#20,H₂O)
  • Electrophoresis (#1A2,#3,#5,#20, #3,#20, H₂O)
  • PCR (2011’s #1,#36 and 2012’s #3,#5,#20, H₂O)

July 20th

  • Miniprep.
  • Electrophoresis (7/19’s PCR product(#1,#3,#5,#20,#36) )
  • Colony PCR (#3,#5,#20)

July 21st

  • Electrophoresis (7/20’s PCR product (#3,#5,#20))


9th week: 7/22-7/28

July 24th

  • Making agar medium plate contain ampicillin
  • PCR (#6, #8)
  • Electrophoresis (#6, #8)
  • Transformation (#1)
  • Culture (#5, #20)

July 25th

  • Miniprep (#5, #20)
  • Purify (#6, #8)
  • Digestion (#3:S-P,#5:E-X,#11:E-S,#20:S-P)
  • Electrophoresis (#5, #11)
  • Ligation (#5,#11) (?)

July 26th

  • Culture (#1)
  • Digestion (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
  • Electrophoresis (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
  • Gel extraction (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)

July 27th

  • Culture, Miniprep (#1)
  • Digestion (#5-A:E-X, #11-A:X-P)
  • Electrophoresis (#3:S-P, #20:S-P, #11:E-S)
  • Gel extraction (#3:S-P, #20:S-P, #11:E-S, E-S vector, hycAp:E-S)

July 28th

ごめんなさい、何やったのかノートからではよく読み取れませんでした；；th ====


10th week: 7/29-8/4

July 30th

  • Digestion (#3:S-P, #11:X-P)
  • Electrophoresis (#3, #11)
  • Gel extraction (#3, #11)
  • Ligation (#3, #11)
  • Transformation (ligation product; #3-11)

August 1st

  • Digestion (#3:S-P, #11:X-P)
  • Electrophoresis (#3, #11)
  • Gel extraction (#3, #11)
  • Ligation (#3, #11)
  • Transformation (ligation product; #3-11)
  • Miniprep (#3, #5, #11)

August 2nd

  • Colony PCR (#3-11)
  • Culture (#3-11)
  • Colony pick up, culture (red colony of #11)
  • Digestion (#3:S-P, #5:E-X)

August 3th

  • Gel extraction (#3:S-P, #5:E-X)
  • Making solution of Kanamycin (100mg/mL)
  • Culture, miniprep (#11)
  • Culture (#11)

August 4th

  • Plating, Miniprep (#11)


11th week: 8/5-8/11

August 6th

  • Culture, Colony PCR (#3-11)

August 7th

  • Colony PCR, culture (#3-11)
  • Miniprep, Digestion, Gel extraction (culture product; #3-11)

August 8th

  • Digestion (#5: E-X)
  • Gel extraction (#5)
  • Miniprep (#5)
  • Colony PCR (culture product; #3-11)

August 9th

  • Colony PCR (#3-11)
  • Culture, Miniprep (#11)
  • Digestion (#11:X-P)
  • Cloning (#6,#8)
  • Ligation
  • Gel extraction (ligation product; #5, #11)
  • Ligation, Transformation (#3, #11)

August 10th

  • Colony PCR (#3-11)
  • Miniprep (#3)
  • Digestion (#3:S-P)
  • Cloning, Colony PCR (#6,#8)
  • cut check, PCR check (#3-11)
  • Electrophoresis (ligation product; #3, #11, PCR product; #6)
  • Gel extraction (#6)

August 11th

  • electrophoresis(PCR product; #3-11)
  • Digestion (#6:E-S)
  • electrophoresis (#8)


12th week: 8/12-8/18


August 13th

  • Check, Culture (#3-11)
  • Transformation (test plasmid)

August 15th

  • Gel extraction (cut check product; #3, #11)
  • Ligation, Transformation (#3-#11)
  • Make Vector for #6
  • Digestion (#3: E-S)
  • Gel extraction (digestion product; #3, #6(8/11)
  • PCR check (ligation product; #3-11)
  • Cloning (#6)

August 16th

  • Gel extraction (#6)
  • cloning (#6)
  • PCR check (#3-11)
  • PCR-cleanup (#8)
  • culture (#3-11)

August 17th

  • electrophoresis (#6)
  • Miniprep (#3-11)
  • Digestion (#3-#11: E-S, #6: E-S)
  • Gel extraction (#3-11)
  • Ligation, Transformation (#3-11-5)
  • Making solution of Ampicillin


11th week: 8/19-8/25


August 20th

  • PCR check (#3-11-5)
  • Digestion (#8: E-S)
  • Cloning (#6)
  • Digeston (#8: E-S)
  • Gel extraction (#6)

August 21th

  • Colony PCR (#1)
  • Digestion (#6: E-S, #3-11: E-S, #20: S-P)
  • Ligation (#3-11, #5)
  • Gel extraction (#3-11, #6)
  • Ligation (#6-pSB1A2)
  • Electrophresis (#3, #5; made previously)

August 22th

  • Cloning (#6; from test plasmid)
  • Colony PCR (#3-11-5(colony is not red), #20, 8/21 ligation product;#6, #8)
  • Miniprep,Frozen stock (culture product; #1, #5, #20)
  • Transformation (#8, #3-11-5)
  • Digestion (#6:X-S)
  • Making solution of Ampicillin
  • cloning retry (#6)

August 23th

  • PCR check (ligation product; #6)
  • Cloning (#6)

Colony PCR (#1, #6, #8, #11, #3-11-5, #3-11)

August 24th

August 25th

This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.


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