Team:SDU-Denmark/labwork/Notebook/week4

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iGEM TEAM ::: SDU-DENMARK

Laboratory Notebook

1st week 2nd week 3th week 4th week
5th week 6th week 7th week 8th week
9th week 10th week 11th week 12th week

23-07-2012 to 29-07-2012

Another checkdigest was made on SST colonies, we had our doubts about the entegrity of the formerly used restriction enzyme, so a newer EcoRI was used, a get was made and this time we got results. After a checkdigest that showed bands at expected lengths and nanodrop, two samples of 1-SST was sent for sequencing. 4 days later we recieved results from sequencing. As we expected from the original ncbi sequences, the genes contained illegal restriction sites. 1-SST contained 1 SpeI restriction site and 1-FFT had 1 EcoRI and 1 XbaI. Primers for mutagenesis were constructed and ordered with the help of our supervisor Steffen Smidth

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