May 29th

• Making LB medium

May 30th

• Making LB Agar
• Making LB Amp Plates

May 31st

• Preculture

June 5th

• Plating JM109

June 6th

• preculture

June 7th

• Measuring OD by spectroscope
• Meking electrocompetent cells
• Electroporating of a test plasmid

June 8th

• Caluculatiing transformation efficiency

June 26th

• Making LB tetracycline plate

June 27th

June 28th

• Making LB kanamycin plate and chloramphenicol plate
• Transformation with a test plasmid

June 29th

• Transformation with #1(constitutive promoter) and #11(RBS)

July 3rd

• Making K solution
• Colony PCR DNA fragment assumed as fhlA (#6) for cloning

July 4th

• Miniprep #1, #11

July 5th

• Making 1% agarose solution
• Electrophoresis (#6)
• Digest (#1_EP, #11-_EP)

July 6th

• Colony PCR (#6)

July 9th

• Electrophoresis (#6)

July 10th

• Transformation
  • #3(RBS medium), #5(d.term), #20(pLac)

July 11th

• Preculture
  • #3, #5, #20
• Gel Extraction(#6)

July 12th

• The continuation of Gel Extraction(#6)
• Miniprep
  • #3, #5, #20

July 13th

• Nanodrop (#6).
• Digestion (#3:E-P, #5:E-P, #20:E-P)
• Electrophoresis (#3, #5, #20)

July 14th

• Miniprep (#1,3,5,11,20)
• Gel extraction (#6)

July 17th

• Transformation (#1A2,#3,#5,#20)
• PCR (parts of 1. and #3,#20(Colony PCR))
• Incubate #5.

July 18th

• Miniprep (#1A2,#3,#5,#20)
• PCR using u.p (#1A2,#3,#5,#20)
• Colony PCR (#3,#20,H₂O)
• Electrophoresis (#1A2,#3,#5,#20, #3,#20, H₂O)
• PCR (2011’s #1,#36 and 2012’s #3,#5,#20, H₂O)

July 20th

• Miniprep.
• Electrophoresis (7/19’s PCR product(#1,#3,#5,#20,#36) )
• Colony PCR (#3,#5,#20)

July 21st

• Electrophoresis (7/20’s PCR product (#3,#5,#20))

July 24th

• Making agar medium plate contain ampicillin
• PCR (#6, #8)
• Electrophoresis (#6, #8)
• Transformation (#1)
• Culture (#5, #20)

July 25th

• Miniprep (#5, #20)
• Purify (#6, #8)
• Digestion (#3:S-P,#5:E-X,#11:E-S,#20:S-P)
• Electrophoresis (#5, #11)
• Ligation (#5,#11) (?)

July 26th

• Culture (#1)
• Digestion (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
• Electrophoresis (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
• Gel extraction (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)

July 27th

• Culture, Miniprep (#1)
• Digestion (#5-A:E-X, #11-A:X-P)
• Electrophoresis (#3:S-P, #20:S-P, #11:E-S)
• Gel extraction (#3:S-P, #20:S-P, #11:E-S, E-S vector, hycAp:E-S)

July 28th

ごめんなさい、何やったのかノートからではよく読み取れませんでした；；th ====

July 30th

• Digestion (#3:S-P, #11:X-P)
• Electrophoresis (#3, #11)
• Gel extraction (#3, #11)
• Ligation (#3, #11)
• Transformation (ligation product; #3-11)

August 1st

• Digestion (#3:S-P, #11:X-P)
• Electrophoresis (#3, #11)
• Gel extraction (#3, #11)
• Ligation (#3, #11)
• Transformation (ligation product; #3-11)
• Miniprep (#3, #5, #11)

August 2nd

• Colony PCR (#3-11)
• Culture (#3-11)
• Colony pick up, culture (red colony of #11)
• Digestion (#3:S-P, #5:E-X)

August 3th

• Gel extraction (#3:S-P, #5:E-X)
• Making solution of Kanamycin (100mg/mL)
• Culture, miniprep (#11)
• Culture (#11)

August 4th

• Plating, Miniprep (#11)

August 6th

• Culture, Colony PCR (#3-11)

August 7th

• Colony PCR, culture (#3-11)
• Miniprep, Digestion, Gel extraction (culture product; #3-11)

August 8th

• Digestion (#5: E-X)
• Gel extraction (#5)
• Miniprep (#5)
• Colony PCR (culture product:#3-11)

August 9th

• Colony PCR (#3-11)
• Culture, Miniprep (#11)
• Digestion (#11:X-P)
• Cloning (#6,#8)
• Ligation
• Gel extraction (ligation product; #5, #11)
• Ligation, Transformation (#3, #11)

August 10th

• Colony PCR (#3-11)
• Miniprep (#3)
• Digestion (#3:S-P)
• Cloning, Colony PCR (#6,#8)
• cut check, PCR check (#3-11)
• Electrophoresis (ligation product; #3, #11, PCR product; #6)
• Gel extraction (#6)

August 11th

• electrophoresis(PCR product; #3-11)
• Digestion (#6:E-S)
• electrophoresis (#8)