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Team:Trieste/notebook
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The Jolly JoCare
a safe probiotic platform for protein expression
Week 1
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Suicide System
We prepared the antibiotics stock ( Amp, Kan, Cm, Spec ) and the DH5-alpha competent cells. We extracted the following BB from the kit: the constitutive promoter ( BB_J23100), the strong RBS (BB_B0034), the weak RBS (BB_B0031), the GFP (BB_E0240) the double terminator (BB_B0015), the toxin Tse2 (BB_K314200). E.coli DH5-alpha competent cells were transformed with 2 ul of each BB extracted. We learnt the safety rules and mostly of the lab techniques.Antibody
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