Team:SEU O China/Notebook
From 2012.igem.org
Notebook
March
2012-3-18 Sun
After discussion in the seminar, 13 students organized the iGEM team and set out to design our project.
2012-3-24 Sat
Our project application were admitted in our school and the registry work started.
The achievements of one week's work:
Muyuan Chen & Xuelong Fan: responsible for the fund application and team registry work.
Xingyu Zhou: designed the bio-sensor based on the asymmetric colony.
Ying Cheng & Zhu Zhu: Searched the information about quorum sensing.
Xiaodan Xu: Read the literature on E.coli's flagella and division.
Peng Cheng & Le Zhou: designed the wiki and logo.
2012-3-25 Sun
Shen Gao: improved the method of cultivation of E.coli.
2012-3-26 Tue
Muyuan Chen: Did some research on light sensor. We voted and named our team SEU_Omega~
2012-3-27 Wed
Filled the application form on the www.igem.org.
2012-3-31 Sat
Xintong Ling: Finished the simulation of diffusion equation in the rectangular case.
April
2012-4-1 Sun
Xintong Ling: finished the diffusion model based on the random containers and made a plan for further models.
2012-4-5 Thur
Muyuan Chen: Made the schedule and experiment program before our summer vacation. Found some information about toggle switch.
Xuelong Fan: Did some research on the light sensor.
Xiaodan Xu: Learnt about how to use NCBI.
2012-4-7 Sat
Had a group meeting and discussed about the future plans.
Xiaodan Xu: Found out the antisense FtsZ gene can be used to control the shape of E.coli's colony.
2012-4-9 Mon
Shen Gao: Choose Pet-32a plasmid for our experiment.
2012-4-12 Thur
Xiaodan Xu: Found a way to verification the primers.
2012-4-13 Fri
Xintong Ling: Finished the simple cell division model.
2012-4-17 Tue
Shen Gao: Learnt about the primer design.
Xingyu Zhou: Designed the quorum sensing part——the AHL system.
Muyuan Chen: Use vectorNTI to analysis plasmid.
2012-4-19 Thur
Xintong Ling:finished the Cellular Automata based on the AHL density.
Xingyu Zhou:Made the expriment plan for the AHL system and found some related kits for his plan.
2012-4-20 Fri
Muyuan Chen: Made the experiment plan for the antisense FtsZ.
Xintong Ling:improved the model of cellular automata.
2012-4-22 Sun
Shen Gao:improved the method of gel extraction and agarose gel electrophoresis.
Xingyu Zhou:Made a summary of AHL sensing system.
2012-4-24 Tue
Xintong Ling & Kai Gui:made the schedule for the further modeling.
Kai Gui:finished the model of biosensor.
2012-4-26 Thur
Xiaodan Xu:Made the experiment plan for antisense FtsZ.
2012-4-29 Sun
Xiaodan Xu:Made a list of the protection base for the restriction sites.
May
2012-5-2 Wed
Xintong Ling: modified the model of biosensor.
2012-5-3 Thur
Xiaodan Xu:Chose the target fragment of FtsZ.
2012-5-7 Mon
Muyuan Chen & Xiaodan Xu: Modified the experiment program--the promoter and the hairpin of the antisense fragment.
2012-5-8 Tue
Ying Cheng: Made the summary of the previous project.
Kai Gui: Finished the model of cellular automata based on the AHL signal diffusion.
2012-5-10 Thur
Our group discussed about the human practice.
Xingyu Zhou:Modified the experiment program of AHL part.
Xintong Ling: Summarise the previous model.
Xiaodan Xu: sorted out the kits list.
2012-5-11 Fri
Xintong Ling:modified the model of cellular automata.
2012-5-12 Sat
Xingyu Zhou:finished the complete AHL experiment program.
2012-5-13 Sun
Xiaodan Xu: Made a list of possibly useful kits.
2012-5-14 Mon
Muyuan Chen: Ordered the paired termini sequence.
2012-5-16 Wed
Xintong Ling & Kai Gui & Jun Zhang: had a meeting talking about the model.
2012-5-22 Tue
Xingyu Zhou: Found out the chemical way of the AHL detection.
2012-5-24 Thur
Xiaodan Xu: transformed the K082034 to make sure the method of Kit usage is correct.
2012-5-27 Sun
Had a group meeting and summarise the work we had done.Made the schedule for the summer vacation and everyone started the preparation for their final examinations.
2012-5-31 Thur Xingyu Zhou: Finished the budget for our project.