Starpoint of the 2nd round of saturated mutagenesis PCR!
Experiment: For the second round of saturated mutagenesis PCR we used the primers Tar6973Fw and Tar6973Rv. Here, aa residues 69 and 73 will be mutated. The PCR protocol is basically the same as for the first round.
Observations and results: The corresponding agarose gel showed a strong band of the expected size (3769 bp).
V07_17
V07_17_1 Test digestion of Miniprep V07_12
Experiment: A test digestion using EcoRI and PstI in a 20 µL batch was performed according to Back to overview
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