Team:TMU-Tokyo/Notebook/Assay

■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)


■Protocols
Plasmid DNA Pure
Genome DNA Purification Restruction Purification
DNA Fragment Ligation
Transformation
Electrophoresis
LB Medium


■Assay
Device1 Assay
Device2 Assay
Device3 Assay

Assay

■Assay1(Device1)

■Assay2(Device2)

■Assay3(Device3)
FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ at 340nm.