Team:Exeter/lab book/proto/2

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Protocol 2


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase

Transferring Cultures to Liquid Medium
Defrost antibiotic for selection, corresponding to the plate antibiotic. Add antibiotic to 5ml of LB broth corresponding to a 1000 fold dilution. Select three colonies from each plate and inoculate to 5ml of LB media via a pipette tip. Incubate cultures overnight in a shaking incubator ~225 rpm, 37 °C.