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From 2012.igem.org

Week 1

Monday, 4th of June

The laboratory work has begun and after preparing this work for several months we are all very excited to finally get it started. Today we started preparing sterile equipment and we also wrote risk declarations for all of our planned experiments. We also introduced ourselves and held a brief presentation of our project to the very nice people working at the department of System’s biology here at Chalmers University of Technology.

Tuesday, 5th of June

Today our dear yeast strains that were kindly provided to us by the Kondo research group of Kobe University, Japan, were transferred to new and fresh YPD agar plates. We also prepared buffers needed for making competent E. coli and we inoculated E. coli DH5α in LB medium.

Wednesday, 6th of June

We prepared a stock of competent E. coli DH5α. Today we also amplified the shuttle plasmids pSP-GM1 and pIYC04 that we will use in our laboratory work, by transforming E. coli DH5α. The cells were then grown on LB-Amp agar plates. We also worked with the team wiki page and created a banner and a logo for the site.

Thursday, 7th of June

Not much laboratory could be done today. Two colonies from each plate containing the E. coli DH5α that were transformed with either pSP-GM1 or pIYC04, were inoculated in ampicillin-containing media and were grown overnight. For the rest of the day, we worked with creating a team brochure and we also established a Facebook page for the team. Click [http://www.facebook.com/ChalmersGothenburgiGEMTeam2012 here] to visit our page and Like our team!

Friday, 8th of June

We purified our pSP-GM1 and pIYC04 plasmids from the transformed E. coli.

Week 2