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From 2012.igem.org

week 17: 20 august - 26 august

Office work

Lab work

Hepatitis B

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20 August

• Colony PCR

- of HepB (without promoter) in BBa_J04450 - of HepB with a his tag + IPTG promoter in Bba_J04500

• Ligation

- of the HepB with the IPTG promoter into BBa_J04450

• Transformation

Both into JM109 and DH5α; using the BBa_J04450 brick as positive control

Hepatitis B inside modification

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GFP modification

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20 August

• Ligation

- of the digested GFP-coil PCR product from 15.Aug into BBa_J04450

• Transformation

Both into JM109 and DH5α; using the BBa_J04450 brick as positive control

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