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Team:Penn/Notebook
From 2012.igem.org
June 2012
June 6th
- Set up some lab equipment
- Autoclaved for a while
- Organized biobrick stuff
- Called Vinoo about DNA planning
June 7th
- Transformed Cph8, pLsr, and LuxS
- Placed order with Vinoo
- Developed idea using PGY/PCN system to activate a gene
June 11th
Wet Lab
- PCR'd mCherry from NAS157
- Ran 1% Gel and purified product
Dry Lab
- Designed primers for LsR promoter
- Meeting with Dr. Sarkar
June 12th
Wet Lab
- Digested mCherry PCR product with BamHI and NotI
- Column purified mCherry and ligated into NAS152 backbone
- Transformed NAS152-mCherry into DH5alpha
- Poured 25 LB-Kan plates
Dry Lab
- Research more information about bacterial drug delivery system
- More research into biofilm project
June 14th
Wet Lab
- Fill in later....
Dry Lab
- Met with Dr. Goulian, obtained pDawn and pDusk
- Identified inaK as a surface display gene we can use
Download
Download the Vallenato script here:
Installation
First, unzip the "vallenato.zip" file and place the "vallenato" folder in the same directory as the html file(s) that will be using the script. If placed in a different folder, you will need to update the paths below.
In the <head> section of your html you will need to link to jQuery, the Vallenato script and stylesheet. You can use the following code:
<script src="http://code.jquery.com/jquery-latest.js" type="text/javascript"></script>
<script src="vallenato/vallenato.js" type="text/javascript"></script>
<link rel="stylesheet" href="vallenato/vallenato.css" type="text/css" media="screen">
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