"

From 2012.igem.org

{{Osaka2}

September 2 (Sun)

1. Colony check
2. miniprep(155(3~6))
3. Tolerance assay(MitomycinC)
4. Colony count
5. Restriction digests(155(3~6))
6. Electrophoresis(155(3~6))
7. To liquid culture(152,1-5A,5-230)
8. Transformation(1-7A(Tet))

September 3 (Mon)

1. miniprep(5-23O,1-5A)
2. Restriction digests(5-230,1-5A)
3. Tolerance assey(MitomycinC)
4. Gel extraction(5-230,1-5A)
5. Ligation(155(1-3A->5-230))
6. Transformation(155,1-7A)

September 4 (Tue)

1. Tolerance assay(MitomycinC)
2. Transformation(155,1-7A,Control)
3. To liquid culture(WT,155,152)

September 5 (Wed)

1. Colony count
2. Tolerance assey(MitomycinC)
3. Transformation(155,BBa_K518010,BBa_K518000)

September 6 (Thu)

1. miniprep(155③④,1-7A①②)
2. Restriction digests(155③④,1-7A①②)
3. Electrophoresis(155③④,1-7A①②)
4. Tolerance assey(MitomycinC)
5. Transformation(sulA promoter)
6. To liquid culture(WT,150,157,158)

September 7 (Fri)

1. Tolerance assey(MitomycinC)
2. miniprep(157①②,158①②)
3. Restriction digests(157,158)
4. Electrophoresis(157,158)
5. Ligation
   • 160(T):158(upstream)+159(downstream)+1-7A(vector)
6. Transformation of ligation products

September 8 (Sat)

1. Restriction digests(149~152)
2. Tolerance assay(MitomycinC)
3. To liquid culture(150,151,159,160)

Back to Notebook