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From 2012.igem.org

Welcome to the NRP UEA iGEM 2012 Wiki Lab Book

Please choose the relevant link to access our diary of that week!

Week Zero | Week One | Week Two | Week Three | Week Four | Week Five | Week Six | Week Seven | Week Eight | Week Nine | Week Ten | Week Eleven | Lab Protocols | Experiments

Contents 1 Week 10 1.1 Day 1 1.2 Day 2 1.3 Day 3 1.4 Day 4 1.5 Day 5

Week 10

Day 1

. As a backup, the running some of the running culture of the Comparator circuit 1 and 2 transformed cells were mini-prepped. After nanodropping, the concentration was found to be.... Following this an overnight double restriction digest with EcoR1 and Pst1 was set up. These were set up using: 0.2 BSA, 2 Buffer H, 0.5 EcoR1 and 0.5 Pst1. As to the DNA, the following amounts were added.

. Following a low concentration reading on Friday, B-M was miniprepped again. However, this produced no DNA when nanodropped. Therefore, B-M was reinoculated into culture.

. Digest MB samples with Spe1 and Pst1, cut out and purify

. CFP and RFP digested with PstI and XbaI (cut out and gell purified)

. Ligate MB2, MB3,MB5,MB9,MB10 with RFP/CFP (overnight)

. Ligate Comparator insert with PSB1C3 (overnight)

Day 2

Day 3

Day 4

Day 5