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23:28, 17 September 2012 (diff | hist) N Team:Washington/Protocols/gene assembly ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} =Gene Synthesis= #Generate Oligo's ##Go to: http://helixweb.nih.gov/dnaworks/ ##Set parameters ###Enter you job title and email ###Ch...")
23:28, 17 September 2012 (diff | hist) N Team:Washington/Protocols/expression purification ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} ='''Protein Expression/Purification of Recombinant Protein in pET29b+ Using Autoinduction'''= __TOC__ Adopted from [http://www.scien...") (top)
23:27, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Kunkel ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} ='''Kunkel Mutagenesis'''= __TOC__ =='''Produce ssDNA (RECOMMENDED TO DO IN DUPLICATE! SEE NOTE AT END OF SECTION 2) '''== *Transfo...") (top)
21:15, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Competent ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} =Competent Cell Stocks= '''CCMB Transformation''' 1. Plate 5ul of cells onto an antibiotic free LB/Agar plate. 2. Incubate over...") (top)
21:15, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Colony ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} =Colony PCR with Green tag= Master mix (7ul): 1ul 10uM forward primer 1ul 10uM reverse Primer 5ul 2x Green tag Cell water (3ul):...") (top)
21:14, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Transformation ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} =General Transformation Protocol= #Thaw 20 μl of frozen competent cells on ice. Do not use glass tubes, which adsorb DNA. #Add 2...") (top)
21:13, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Ligation ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} =General Ligation Protocol= 7uL insert 1uL vector 1uL T4 ligase buffer 1uL T4 ligase Incubate at 16C overnight, or room temperat...") (top)
21:12, 17 September 2012 (diff | hist) N Team:Washington/Protocols/Digestion ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} =General Digestion Protocol= 10 uL DNA 5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of en...")
21:11, 17 September 2012 (diff | hist) N Team:Washington/Protocols/PCR ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} =General PCR Protocol= #Setup Amplification PCR Reaction ##1uL Template ##1uL 25mM dNTP's ##10uL Phusion HF Buffer ##0.5uL Forward P...")
21:04, 17 September 2012 (diff | hist) N Team:Washington/Protocols/gel electrophoresis ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} =Agarose Gel Electrophoresis= == General Procedure == #Cast a gel #Place it in gel box in running buffer #Load samples #Run the gel...")
20:27, 17 September 2012 (diff | hist) Team:Washington/Protocols ‎
23:03, 13 September 2012 (diff | hist) N Team:Washington/Protocols ‎ (Created page with "<h1 id='General'>General Protocols</h1>")
22:55, 13 September 2012 (diff | hist) Team:Washington/Plastics ‎
22:54, 13 September 2012 (diff | hist) Template:Team:Washington/Templates/Top ‎
22:53, 13 September 2012 (diff | hist) N Team:Washington/Flu ‎ (Created page with "{{Template:Team:Washington/Templates/Top}} __NOTOC__ <h1 id='Background'>Background</h1> stuff goes here YEAAAH<br> stuff goes here YEAAAH<br> stuff goes here YEAAAH<br> stuff ...")
22:50, 13 September 2012 (diff | hist) Team:Washington/Plastics ‎
22:49, 13 September 2012 (diff | hist) Team:Washington/Optogenetics ‎
22:47, 13 September 2012 (diff | hist) Team:Washington/Optogenetics ‎
22:46, 13 September 2012 (diff | hist) Team:Washington/Optogenetics ‎
22:45, 13 September 2012 (diff | hist) Team:Washington/Optogenetics ‎
22:39, 13 September 2012 (diff | hist) N File:Washington nick bolten.jpg ‎ (top)
22:26, 13 September 2012 (diff | hist) Team:Washington/Team:Members ‎
22:25, 13 September 2012 (diff | hist) N File:Aaron bike UW.png ‎ (top)
22:15, 13 September 2012 (diff | hist) Team:Washington/Team:Members ‎
22:13, 13 September 2012 (diff | hist) N File:Washington erik murphy.JPG ‎ (top)
22:11, 13 September 2012 (diff | hist) N File:Washington max gelb.jpg ‎ (top)
22:10, 13 September 2012 (diff | hist) N File:Washington ruihong wang.jpg ‎ (top)
22:09, 13 September 2012 (diff | hist) N File:Washington bill fu.jpg ‎ (top)
22:00, 13 September 2012 (diff | hist) N File:Washington felix ekness.jpg ‎ (top)
01:13, 12 September 2012 (diff | hist) Team:Washington ‎
00:43, 12 September 2012 (diff | hist) Team:Washington ‎
00:38, 12 September 2012 (diff | hist) Team:Washington ‎
00:37, 12 September 2012 (diff | hist) Team:Washington ‎
00:23, 12 September 2012 (diff | hist) N File:FriendlyPicture.jpg ‎ (top)
00:18, 12 September 2012 (diff | hist) Team:Washington ‎
00:16, 12 September 2012 (diff | hist) Team:Washington ‎
00:40, 7 September 2012 (diff | hist) Team:Washington/Safety ‎
00:25, 7 September 2012 (diff | hist) Team:Washington ‎
22:47, 4 September 2012 (diff | hist) Team:Washington ‎
22:45, 4 September 2012 (diff | hist) Team:Washington ‎
21:06, 4 September 2012 (diff | hist) Team:Washington ‎
00:08, 29 August 2012 (diff | hist) Team:Washington ‎
23:23, 28 August 2012 (diff | hist) Team:Washington/googlea7ec35b23b91ed35.html ‎ (Blanked the page) (top)
23:22, 28 August 2012 (diff | hist) N Team:Washington/googlea7ec35b23b91ed35.html ‎ (Created page with "googlea7ec35b23b91ed35")
23:06, 28 August 2012 (diff | hist) Team:Washington ‎
22:59, 28 August 2012 (diff | hist) N File:IGEM Cover Photo.jpg ‎ (A few members of the UW iGEM team--current placeholder.) (top)
22:54, 28 August 2012 (diff | hist) Team:Washington ‎
22:54, 28 August 2012 (diff | hist) Team:Washington ‎
22:42, 28 August 2012 (diff | hist) Team:Washington ‎
22:02, 28 August 2012 (diff | hist) Team:Washington ‎

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