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Team:SDU-Denmark/labwork/Notebook/week2
From 2012.igem.org
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<pre>30 cycles | <pre>30 cycles | ||
95°C for 30 seconds | 95°C for 30 seconds | ||
| - | Annealing temperature | + | Annealing temperature (5°C below primer melting temperature) |
59°C for 20 seconds</pre>72°C for 3 minutes</pre> | 59°C for 20 seconds</pre>72°C for 3 minutes</pre> | ||
Revision as of 08:28, 7 September 2012
Laboratory Notebook
Here you find the log book for the procedures carried out in the laboratory, starting from week 27.
06-07-2012:
PCR of SST and FFT
We did a polymerase chain reaction with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers.
PCR program:
95°C for 3 minutesThe genomic template we used was MCF7. The PCR samples were processed over night (O.N.).30 cycles 95°C for 30 seconds Annealing temperature (5°C below primer melting temperature) 59°C for 20 seconds72°C for 3 minutes
