Team:SDU-Denmark/labwork/Notebook/week2

From 2012.igem.org

(Difference between revisions)
Line 293: Line 293:
<pre>30 cycles
<pre>30 cycles
95°C for 30 seconds  
95°C for 30 seconds  
-
53°C for 20 seconds
+
Annealing temperature
-
56°C for 20 seconds
+
59°C for 20 seconds</pre>72°C for 3 minutes</pre>
-
59°C for 20 seconds</pre>  
+
-
72°C for 3 minutes</pre>
+
The genomic template we used was MCF7. The PCR samples were processed over night (O.N.).
The genomic template we used was MCF7. The PCR samples were processed over night (O.N.).

Revision as of 08:26, 7 September 2012

iGEM TEAM ::: SDU-DENMARK

Laboratory Notebook

Here you find the log book for the procedures carried out in the laboratory, starting from week 27.

1st week 2nd week 3th week 4th week
5th week 6th week 7th week 8th week

06-07-2012:

PCR of SST and FFT


We did a polymerase chain reaction with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers.
PCR program: 

95°C for 3 minutes 

30 cycles
95°C for 30 seconds 
Annealing temperature 
59°C for 20 seconds
72°C for 3 minutes
The genomic template we used was MCF7. The PCR samples were processed over night (O.N.).

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