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Team:SDU-Denmark/labwork/Notebook/week2
From 2012.igem.org
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We did a <a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/PCR"><b>polymerase chain reaction</b></a> with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers. <br> | We did a <a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/PCR"><b>polymerase chain reaction</b></a> with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers. <br> | ||
PCR program: </br> | PCR program: </br> | ||
| - | + | <pre>95°C for 3 minutes | |
| + | <pre>30 cycles | ||
| + | 95°C for 30 seconds | ||
| + | 53°C for 20 seconds | ||
| + | 56°C for 20 seconds | ||
| + | 59°C for 20 seconds</pre> | ||
| + | 72°C for 3 minutes | ||
| + | </pre> | ||
The genomic template we used was MCF7. The PCR samples were processed over night (O.N.). | The genomic template we used was MCF7. The PCR samples were processed over night (O.N.). | ||
Revision as of 08:21, 7 September 2012
Laboratory Notebook
Here you find the log book for the procedures carried out in the laboratory, starting from week 27.
06-07-2012:
PCR of SST and FFT
We did a polymerase chain reaction with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers.
PCR program:
95°C for 3 minutesThe genomic template we used was MCF7. The PCR samples were processed over night (O.N.).30 cycles 95°C for 30 seconds 53°C for 20 seconds 56°C for 20 seconds 59°C for 20 seconds72°C for 3 minutes
